In 1991, eight people sealed themselves into an artificial enclosed ecosystem in Arizona; oxygen concentrations dropped from 21% to 14% within 16 months after carbon dioxide became locked into the facility's concrete surfaces
Eight individuals entered Biosphere 2 in 1991, aiming to create a self-sustaining miniature Earth. However, within 16 months, oxygen levels plummeted from 21% to 14%. Scientists discovered that soil microorganisms consumed oxygen during respiration, and the resulting carbon dioxide was absorbed by the structure's concrete, preventing atmospheric replenishment. This unforeseen interaction highlighted the immense complexity of closed ecosystems.
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